Figure 2.

Muscle weight and histological changes of mice fed with HFD for 12 weeks. (A) Quadriceps and (B) gastrocnemius muscle weight in mice (n = 3–5). (C) Quadriceps weight or (D) gastrocnemius weight as a percentage of total body weight are calculated (n = 3–5). (E) Representative hematoxylin and eosin staining of quadriceps in mice (n = 3–5) are shown (scale bar, 50 μm). (F) Quantification of mean muscle fiber diameters was performed by Image J software (n = 3–5). The data are presented as the mean ± SEM, unpaired two-tailed Student's t-test. *P < 0.05, **P < 0.01.