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Lane 1, TEM-4 (pI = 5.9) and SHV-1 (pI = 7.6) β-lactamases; lane 2, SHV-3 (pI = 7.0); lanes 3 and 4, Ravasio (BRO-1) and 1908 (BRO-2) control strains, respectively; lanes 5 and 7 and 9 to 13, clinical isolates showing BRO-1 banding patterns; lanes 6 and 8, clinical strains showing BRO-2 banding patterns; lane 14, a β-lactamase-negative isolate.
