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. Author manuscript; available in PMC: 2022 Mar 31.
Published in final edited form as: Neurobiol Aging. 2013 Jan 16;34(6):1540–1548. doi: 10.1016/j.neurobiolaging.2012.12.011

Fig. 2.

Fig. 2.

Expression of FKN constructs. (A) Western blot with anti-HA tag antibody using cell lysates of plasmid transfected HEK293 cells. (B) Anti-HA Western blot of the media from transfected HEK293 cells, only sFKN is observed in the media. (C and D) Anti-HA immunohistochemistry of hippocampus of GFP-injected and sFKN-injected mice. Scale = 200 μm. Abbreviations: DG, dentate gyrus; FKN, fractalkine; GFP, green fluorescent protein (control); HA, hemagglutinin; n, native (membrane associated) FKN; sFKN, secreted FKN.