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. 2022 Mar 17;9:860086. doi: 10.3389/fnut.2022.860086

Table 2.

The primers for quantitative real-time PCR.

Gene Gene ID Primer Sequence (5-3) Size (bp)
OPG XM_005017709.3 Reverse gcctaactggctgaacttgc 106
Forward gaaggtctgctcttgcgaac
RANKL XM_021276016.1 Reverse gccttttgcccatctcatta 100
Forward taagtttgcctggcctttgt
ZO-1 XM_013104939.1 Reverse tacgcctgtgaagaatgcag 86
Forward ggagtggtggtgtttgcttt
Occludin XM_013109403.1 Reverse caggatgtggcagaggaatacaa 160
Forward ccttgtcgtagtcgctcaccat
Claudin 1 XM_013108556.1 Reverse tcatggtatggcaacagagtgg 179
Forward cgggtgggtggataggaagt
Malt1 XM_027446455.2 Reverse ccatggaaaccgtacttgct 118
Forward ttgtgcaggggattggtaat
NF-κB XM_027455993.1 Reverse gagcgttttcaagaggttgc 123
Forward agggatcttctcctgccatt
TNF-α EU375296.1 Reverse agatgggaagggaatgaacc 51
Forward gttggcataggctgtcctgt
IL-1β DQ393268.1 Reverse gcatcaagggctacaagctc 131
Forward caggcggtagaagatgaagc
IL-6 AB191038.1 Reverse atctggcaacgacgataagg 87
Forward ttgtgaggagggatttctgg
IL-17 EU366165.1 Reverse atgcctgacccaaaaagatg 145
Forward gtggtcctcatcgatcctgt
IL-18 XM_027444356.2 Reverse ctgatgacgatgagctggaa 120
Forward caaaagctgccatgttcaga
GPR41 KJ523111.1 Reverse actgacgtcctcctcctcaa 160
Forward tggtgaggtagatgctggtg
GPR43 KJ523110.1 Reverse agcagctgagctttgtcctc 129
Forward gtggaatattaggccgagca
NaPi-IIb NM_204474.3 Reverse gctccagcacttcttcatcc 95
Forward aatgtttgcccccataatga
Calbindin 1 XM_027452451.2 Reverse cagggtgtcaaaatgtgtgc 109
Forward gatccttcagcagtgcatca
β-actin NM_001310408.1 Reverse ccagccatctttcttgggta 105
Forward gtgttggcgtacaggtcctt
GAPDH XM_005016745.3 Reverse tttttaaccgtggctccttg 94
Forward actgggcatggaagaacatc

OPG, osteoprotegerin; RANKL, receptor activator of nuclear Factor-κ B ligand; ZO-1, zonula occludens; Malt1, mucosa-associated lymphoid tissue lymphoma translocation protein 1; NF-κB, nuclear factor kappa B; TNF-α, tumor necrosis factor alpha; IL, interleukin; GPR, G protein-coupled receptor; NaPi-IIb, sodium-dependent phosphorus transport protein IIb; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.