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. 2022 Mar 28;9(2):ENEURO.0476-21.2022. doi: 10.1523/ENEURO.0476-21.2022

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Copyright © 2022 Wiggin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 1. — V3-INs are identified by co-localization of vglut2a:DsRed and nkx2.2a:mEGFP transgene expression in neurons located in the ventromedial spinal cord. A, Whole-mount Tg(nkx2.2a:mEGFP)^vu17;Tg(vglut2a:DsRed)^nns9 double transgenic zebrafish larva. B–D, A representative single confocal optical section of the spinal cord exhibiting: (B) nkx2.2a:mEGFP expression, (C) vglut2a:DsRed expression, and (D) overlayed nkx2.2a:mEGFP and vglut2a:DsRed expression. The boundaries of the spinal cord and central canal are illustrated by solid and dashed white lines, respectively, in panel B. White triangles indicate neurons that co-express both transgenes and the black triangle indicates the only vglut2a:DsRed neuron ventral to the central canal that did not co-express nkx2.2a:mEGFP in the field of view. Scale bars: 1 mm (A) and 10 μm (B–D).