Figure 4.

EHD1 localization in relation to cubilin and megalin. In wildtype mice (upper row), Ehd1 (green) was predominantly localized in the subapical compartment. Cubilin is shown in red, megalin in blue, and cell nuclei (DAPI) in magenta. In knockout mice (middle row) and knockin mice (lower row), the localization of cubilin and megalin appeared unaffected. In knockin mice, Ehd1 abundance was reduced and mutant Ehd1 was observed in intracellular tubules. Left four panels are confocal images; right high magnification panel: stimulated emission depletion microscopy image for Ehd1, cubilin, and megalin. Deconvolution of all images was performed using Huygens software. Scale bar: 5 µm.