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. 2022 Mar 21;18(3):e1010131. doi: 10.1371/journal.pgen.1010131

Fig 2. Construction of BmPMFBP1 mutants using the binary transgenic CRISPR/Cas9 system.

Fig 2

(A) Schematic of the BmPMFBP1 gene structure and sgRNA target sites. The boxes indicate the coding exon, the black arrows indicate the start and stop codon, the red arrows indicate the two target sites located in exons 1 and exons 2. (B) Schematic of the binary transgenic CRISPR system vectors for obtaining BmPMFBP1 mutants. (C) The mRNA expression level of BmPMFBP1 in three individual WT and mutant testes at L5D4. The asterisks (***) indicate the significant differences (P < 0.001) relative to WT. (D) Examples of mutations induced by CRISPR/Cas9 system. The sequence of the wildtype is displayed at the top. The dotted lines indicate the deleted residues, the PAM sequence is in red, the number of the nucleotides deleted are shown on the right.