Fig 3. BmPMFBP1 deficiency results male sterility.

(A) Photograph of eggs after 8 days laid by wildtype or mutant females mated with wildtype or mutant males. The color of eggs indicated whether the egg is fertilized. Pale yellow eggs are unfertilized, dark black eggs are successfully fertilized. (B) Fertility of BmPMFBP1 male mutants. Fertility was evaluated as the ratio of fertile individuals to the total number of individuals (n = 20). (C) The cumulative amounts of eggs laid by female. Data are mean ± SEM (n = 30, ns indicates no significant difference compared with WT Virgin, P>0.05; *** indicates significant difference compared with the WT Virgin, P<0.001)). (D) Morphologies of internal structure of wildtype (left) and BmPMFBP1 mutant (right) testis. The red triangles indicate the nuclei of eupyrene sperm bundles.