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The eupyrene sperm bundles showed abnormal location and spacing of nuclei in ΔBmPMFBP1 males. (A) Fluorescence image of eupyrene sperm bundles in testes of wildtype (WT) males and ΔBmPMFBP1 males on the seventh day of pupal stage. The filamentous actin proteins were stained with TRITC Phalloidin, the nuclei were stained with Hoechst 33258. (B) Additional image of eupyrene bundles with fluorescence staining for nuclei.
