Table 1. SARS-CoV-2 tissue distribution following intranasal inoculation in white-tailed deer.
| assay | real-time reverse transcriptase PCR | virus isolation/titration | in situ hybridization | immunohistochemistry | ||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| group | inoculated | control | Inoculated | control | Inoculated | control | Inoculated | control | ||||||||||||||||||||||||
| euthanasia day | 2 pi | 5 pi | 20 pi | 2 pi | 5 pi | 20 pi | 2 pi | 5 pi | 20 pi | 2 pi | 5 pi | 20 pi | ||||||||||||||||||||
| deer ID | #1748 | #1758 | #1703 | #1842 | #1705 | #1810 | #1754 | #1815 | #1748 | #1758 | #1703 | #1842 | #1705 | #1810 | #1754 | #1815 | #1748 | #1758 | #1703 | #1842 | #1705 | #1810 | #1754 | #1815 | #1748 | #1758 | #1703 | #1842 | #1705 | #1810 | #1754 | #1815 |
| palatine tonsil | + | + | + | + | + | + | - | - | + | + | + | + | - | - | nt | nt | + | + | + | + | nt | nt | nt | nt | + | + | + | + | nt | nt | - | nt |
| pharyngeal tonsil | + | + | + | + | + | + | - | - | + | + | + | + | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
| nasal turbinates | + | + | + | + | + | + | - | - | + | + | + | + | - | - | nt | nt | + | + | + | + | nt | nt | nt | nt | + | + | + | + | nt | nt | - | nt |
| retropharyngeal LN | + | + | + | + | + | + | - | - | + | + | + | + | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | + | + | + | + | nt | nt | - | nt |
| mandibular LN | + | + | + | + | + | + | - | - | + | + | + | + | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | + | + | + | + | nt | nt | - | nt |
| cerebellum | + | + | + | - | - | + | - | - | + | + | - | nt | nt | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | - | - | - | - | nt | nt | - | nt |
| cerebrum | + | + | + | + | + | + | - | - | + | + | + | - | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | |
| olfactory lobes | + | + | + | + | + | + | - | - | + | + | + | + | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | + | + | + | + | nt | nt | - | nt |
| parotid salivary gland | + | + | + | + | + | + | - | - | - | - | - | - | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
| caudate nucleus of brain | + | + | + | + | + | - | - | - | + | + | - | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | - | - | - | - | nt | nt | - | nt |
| thymus | + | + | + | - | - | - | - | - | - | + | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
| trachea | + | + | + | + | + | - | - | - | + | + | + | + | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | + | - | - | - | nt | nt | - | nt |
| bronchus | + | + | + | + | + | + | - | - | + | + | + | + | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
| lung | + | + | + | - | - | - | - | - | + | + | + | nt | nt | nt | nt | nt | + | + | + | + | nt | nt | nt | nt | + | + | + | - | nt | nt | - | nt |
| heart | + | + | - | + | - | - | - | - | - | - | nt | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
| tracheobronchial LN | + | + | + | + | - | - | - | - | - | + | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
| mediastinal LN | + | + | + | + | + | - | - | - | - | - | - | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
| lung | - | - | - | + | - | - | - | - | nt | nt | nt | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
| spleen | - | - | - | - | - | - | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
| kidney | - | - | - | - | - | - | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
| ileum | + | - | - | - | - | - | - | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
| ileocecal valve | + | + | - | + | - | - | - | - | - | - | nt | + | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
| spiral colon | + | - | - | - | - | - | - | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
| mesenteric LN | + | - | - | - | - | - | - | - | - | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt | nt |
"+" = detected; "-" = undetected; "nt" = not tested; LN: lymph node