Table 2. Kinetic parameters of human GFPT1 and GFPT2.

	L-Glu production			D-GlcN6P production			UDP-GlcNAc inhibition
	Km L-Gln[mM]	kcat[s–1]	kcat/Km[mM–1 s–1]	Km Frc6P[mM]	kcat[s–1]	kcat/Km[mM–1 s–1]	IC50[µM]
GFPT1	1.1±0.19	3.6±0.18	3.3	0.08±0.01	1.7±0.09	21.3	57.0–8.3/+9.7
GFPT2	0.5±0.06	3.7±0.10	7.4	0.29±0.05	1.8±0.09	6.2	367.3–43.6/+49.5
Unpaired t-test(two-sided)	**p=0.005			**p=0.0027			***p=0.0002