Table 2.

Characterization of 1α,25(OH)₂D Binding to CYP24A1 in the Presence of Adx Mutants^a

	KD (μM)	ΔAmax (AU)
hCYP24A1
no Adx	1.1 ± 0.2	0.03 ± 0.005
WT	1.5 ± 0.2	0.03 ± 0.004
D72N_D76N	0.7 ± 0.1	0.02 ± 0.002
L80Q	0.8 ± 0.1	0.02 ± 0.001*
L80V	0.6 ± 0.05	0.04 ± 0.003*
L80I	0.8 ± 0.08	0.04 ± 0.003*
rCYP24A1
no Adx	0.3 ± 0.01	0.05 ± 0.004
WT	0.3 ± 0.03	0.07 ± 0.003**
L80V	0.4 ± 0.03	0.07 ± 0.004**

^aOne-way ANOVA analysis is indicated by one asterisk (P ≤ 0.05) or two (P ≤ 0.01).