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. 2022 Jan 20;41(2):66–72. doi: 10.12938/bmfh.2021-067

Table 2. Level of miR-669c-3p expression in the liver measured by quantitative RT-PCR.

Average Cta Average Cta The average of ΔCtb
(U6) (miR-669c-3p) (Ct(miR-669c-3p) – Ct(U6))
CTL 22.74 ± 0.11 31.28 ± 0.12 8.54 ± 0.13
SE 22.54 ± 0.06 31.54 ± 0.15 9.00 ± 0.17*

CTL: control; SE: sesame lignans.

aCt is the number of cycles that fluorescence passes the threshold in the amplification curve.

bΔCt = Ct (miR-669c-3p) – Ct (U6). Data are presented as the mean ± SEM (n=12). *p<0.05 by two-tailed Student’s t-test.