Table 2. Level of miR-669c-3p expression in the liver measured by quantitative RT-PCR.
| Average Cta | Average Cta | The average of ΔCtb | |
|---|---|---|---|
| (U6) | (miR-669c-3p) | (Ct(miR-669c-3p) – Ct(U6)) | |
| CTL | 22.74 ± 0.11 | 31.28 ± 0.12 | 8.54 ± 0.13 |
| SE | 22.54 ± 0.06 | 31.54 ± 0.15 | 9.00 ± 0.17* |
CTL: control; SE: sesame lignans.
aCt is the number of cycles that fluorescence passes the threshold in the amplification curve.
bΔCt = Ct (miR-669c-3p) – Ct (U6). Data are presented as the mean ± SEM (n=12). *p<0.05 by two-tailed Student’s t-test.