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. 2022 Apr 1;132(7):e156507. doi: 10.1172/JCI156507

Figure 2. Preventive anti-CD45RC mAb immunotherapy decreases the production of autoantibodies in Aire–/– rats.

Figure 2

(A) Tissue sections of organs from IgM–/– rats were incubated with sera of Aire–/– animals at 4 months of treatment with anti-CD45RC or isotype control mAb. Autoantibodies are depicted in yellow and DAPI in blue. Original magnification, ×20. Images are representative of 3 different experiments. (B) Sera from anti-CD45RC or isotype control mAb–treated Aire–/– rats at 4 months of treatment were incubated on Western blot membranes after migration and transfer of tissue-specific self-antigens from IgM–/– rats. Binding of autoantibodies was revealed using an anti-rat IgG biotin-coupled antibody and avidin peroxidase. β-Actin was used as a loading control. Data are representative of 5 different experiments. MLN, mesenteric lymph nodes. (C) Anti-cytokine and tissue-specific autoantibodies were quantified by LIPS assay using sera from anti-CD45RC or isotype control mAb–treated Aire–/– rats at 4 months of treatment. Normalization was achieved by division of the obtained value by the mean values obtained from WT animals. Mann-Whitney analysis showed no significant difference between the 2 groups.