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. 2022 Apr 1;132(7):e154422. doi: 10.1172/JCI154422

Figure 10. Nef activity is higher in HIV-1–infected CD4+ Tem cells.

Figure 10

Memory CD4+ T cells from HIV-1– donors were sorted and infected with (A and B) HIV-BaL or (C–E) HIV-NL4-3-eGFP for 5 days. (A) Representative dot plots (top) and histograms (bottom) of 10 independent experiments showing CD4 geometric mean fluorescence intensity (gMFI) in uninfected p24– and HIV-1–infected p24+ cells. (B) CD4 expression in HIV-1–infected cells was calculated as CD4 gMFI in p24+ cells relative to CD4 gMFI in p24– cells. (C) Representative dot plots (top) and histograms (bottom) of 4 independent experiments showing HLA-A*02 gMFI in uninfected eGFP– and HIV-1–infected eGFP+ cells. (D) HLA-A*02 downmodulation in HIV-1–infected cells was calculated as HLA-A*02 gMFI in eGFP+ cells relative to HLA-A*02 gMFI in eGFP– cells. (E) eGFP MFI relative to Tcm values. Data represent the mean ± SD (B) or median ± 95% CI (D and E). One-way ANOVA followed by the (B) Tukey’s HSD post test or (D and E) Kruskal-Wallis followed by Dunn’s post test were performed to determine statistical significance. Each data point represents a single donor. Tcm, central memory; Ttm, transitional memory; Tem, effector memory. *P ≤ 0.05, **P ≤ 0.01.