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. 2022 Apr 1;132(7):e154422. doi: 10.1172/JCI154422

Figure 8. Nef– HIV-1 induces higher CD8+ T cell activity.

Figure 8

CD4+ T cells isolated from HIV-1+ donors were infected with HIV-WT or HIV-ΔNef and cocultured with autologous CD8+ T cells. (A) Timeline illustrating the experimental procedure, as described in Methods. (B) Flow cytometry of CD107a/b expression in CD8+ T cells from a representative experiment. (C) Expression of CD107a/b relative to values obtained from CD8+ T cells cocultured with HIV-WT–infected cells. Data represent mean ± SD. Statistical significance was determined by 2-tailed Student’s t test. Each experiment was performed in triplicate. **P ≤ 0.01. (D) Representative flow cytometry of 2 independent experiments showing p24 expression of cells infected with HIV-WT or HIV-ΔNef with or without coculture with autologous CD8+ T cells. (E) p24 values of cells infected with HIV-WT or HIV-ΔNef after 6 and 24 hours of coculture with CD8+ T cells relative to p24 expression in the CD4+ T cell–only condition.