FIGURE 3.

Effect of detergent lysis with Triton X‐100 on ApoB‐containing lipoproteins co‐stained with Anx5, lactadherin and CD41. (a) Scatter plots of Anx5‐Cy5 and lactadherin‐FITC versus ApoB‐PE fluorescence of a representative specifically stained fresh PPP sample and its detergent lysis controls depicting the degree to which the different fluorescently labelled populations are affected by detergent lysis. Fluorescence intensities is denoted in arbitrary units. (b) Bar plots of the concentrations of different marker‐positive populations in specifically stained fresh PPP (blue) and detergent lysis controls (red). Bar plots for fresh PPP depict the mean and standard deviation (error bars) for three individuals. Anx5: Annexin V; ApoB: Apolipoprotein B; CD41: Cluster of differentiation 41/platelet membrane glycoprotein IIb‐IIIa complex; Lact: Lactadherin