Figure 3.

LLSOs change both the expression and localization of E-cadherin in PC3 cells

(A) Western blot for E-cadherin expression was performed with proteins extracted following 48-h treatments with DMSO and LLSOs in PC3 cells. Quantification of the E-cadherin blot is shown in the histogram on the right. All 3 chemicals showed an increase in the E-cadherin expression level. Normalization of the E-cadherin signal is done with the GelEZ system (Bio-Rad), which quantifies total protein on the transfer membrane (left lower panel). The experiment was repeated 3 times. (B) Immunofluorescence analysis was performed following 48-h treatments with DMSO and LLSOs in PC3 cells. PC3 cells stained with mouse IgG were used as a negative control and LNCaP cells were used as a positive control. All 3 chemicals induce an increase in E-cadherin expression levels as well as a more junctional localization. Representative examples from at least 10 microscopic fields per experiment, and experiments repeated 3 times. Photomicrographs were taken at 1,000× magnification; scale bar is 15 μm.