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. 2022 Mar 31;13(3):288. doi: 10.1038/s41419-022-04744-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Hoechst staining revealed typical morphologic changes during apoptosis after treatment with 30 μM AMTB for 24 h. B, C Annexin V/PI staining revealed a significant increase in apoptosis rate in 143B cells and U2OS cells after incubation with AMTB for 24 h. The data was compared by one-way ANOVA with Tukey’s multiple comparison test and shown as mean ± s.d. **P < 0.01. D, E The level of cleaved caspase-3 and cleaved PARP was examined by western blot assay and an increased cleavage of caspase-3 and PARP was observed in a time- and dose-dependent manner. Western blot is representative of three independent western blot with different lysates.