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. 2022 Mar 31;8:36. doi: 10.1038/s41378-022-00370-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Phase-contrast and H&E staining assessment of the cell-laden chips after 6 days of coculture with continuous medium perfusion. b, c Immunostaining of PCCs for EpCAM, U937 cells for CD68, and PSCs for α-SMA. The expression profile of human U937 monocytes and PSCs in the organoids was performed by qPCR analysis. The fold change in gene expression was normalized to the housekeeping gene GAPDH and compared to the cells in monoculture. Data were expressed as the mean ± SEM, n = 3 per experiment. Scale bar, 100 µm. *p < 0.05, **p < 0.01, ***p < 0.001