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. 2022 Mar 31;6:19. doi: 10.1038/s41698-022-00262-y

Table 2.

Univariable and multivariable Cox regression analysis of miRisk and clinical covariates.

Overall survival Univariable analysis Multivariable analysis
Covariate HR 95% CI p-value HR 95% CI p-value
Training cohort
PD-L1 (≥50%) 1.81 1.04–3.16 0.036 1.48 0.69–3.17 0.314
ECOGa (>0) 1.49 0.84–2.64 0.175 1.41 0.75–2.66 0.284
Gender (male vs female) 0.82 0.48–1.40 0.457 0.86 0.48–1.56 0.623
Age (>75 years) 1.27 0.67–2.41 0.470 1.25 0.58–2.69 0.577
Therapy line incl. <IV (>2) 1.43 0.35–5.93 0.621 1.61 0.34–7.58 0.549
Substance (pembro vs nivo) 0.60 0.33–1.07 0.085 0.96 0.44–2.10 0.926
Histology (non-adeno vs adeno) 1.19 0.70–2.04 0.519 1.22 0.68–2.19 0.513
Smoking status (ever smoker vs other) 0.67 0.29–1.57 0.356 0.68 0.24–1.94 0.472
ANCb (>7.5) 1.87 1.09–3.22 0.023 1.24 0.68–2.27 0.485
ALCc (>1) 0.61 0.35–1.04 0.068 0.88 0.47–1.65 0.683
miRisk (high vs low) 4.35 2.41–7.85 <0.001 3.83 1.99–7.37 <0.001
Independent validation cohort
PD-L1 (≥50%) 1.67 0.95–2.94 0.073 1.54 0.45–5.31 0.492
ECOGa (>0) 2.82 1.47–5.41 0.002 4.46 2.15–9.24 <0.001
Gender (male vs female) 1.07 0.6–1.93 0.816 0.81 0.43–1.52 0.508
Age (>75 years) 1.66 0.85–3.24 0.141 1.81 0.87–3.81 0.115
Therapy line incl. <IV (>2) 1.36 0.73–2.54 0.327 1.32 0.67–2.58 0.421
Substance (Pembro vs Nivo) 0.68 0.39–1.19 0.176 1.18 0.35–4.01 0.789
Histology (Non-adeno vs adeno) 1.63 0.93–2.85 0.088 1.59 0.81–3.12 0.174
Smoking status (ever smoker vs other) 1.92 0.46–7.96 0.367 4.53 0.98–20.99 0.054
ANCb (>7.5) 1.98 1.08–3.62 0.027 1.06 0.54–2.11 0.862
ALCc (>1) 0.57 0.32–0.99 0.047 0.68 0.38–1.22 0.200
miRisk (high vs low) 2.47 1.36–4.48 0.003 2.49 1.16–5.34 0.019
Control cohort
PD-L1 (≥50%) 1.46 0.64–3.32 0.364 1.59 0.65–3.87 0.307
ECOGa (>0) 1.66 0.85–3.25 0.136 2.13 1.05–4.35 0.037
Gender (male vs female) 1.14 0.59–2.20 0.698 1.06 0.52–2.16 0.878
Age (>75 years) 0.75 0.27–2.12 0.590 0.52 0.17–1.56 0.241
Histology (Non-adeno vs adeno) 0.93 0.42–2.03 0.851 0.69 0.31–1.53 0.360
Smoking status (ever smoker vs other) 0.90 0.28–2.91 0.854 0.76 0.21–2.68 0.664
ANCb (>7.5) 0.47 0.25–0.90 0.022 0.37 0.18–0.75 0.006
ALCc (>1) 0.96 0.50–1.82 0.893 0.91 0.45–1.82 0.787
miRisk (high vs low) 1.36 0.70–2.64 0.369 2.52 1.20–5.30 0.015

aEastern Cooperative Oncology Group (ECOG) performance status.

bAbsolute neutrophil count.

cAbsolute lymphocyte count.