Table 3.

PCR and sequencing primers and PCR cycling conditions for cpDNA matK, rbcL-accD IGS, trnK–rps16 IGS, ycf6-psbM IGS and psbA-trnH IGS, and nDNA pgiC. Superscripts following primer names denote reference numbers. Primers pgiC_16F (5'-CAC AGC TTT TAC CAA CTG ATT C-3') and pgiC_21R (5'- CCT AAC TCA ACT CCC CAC-3') were designed by the authors.

Region	Primer	PCR cycling condition (35 cycles)
		Predenaturation (3 min) (°C)	Denaturation (1 min) (°C)	Annealing (40 s) (°C)	Extension (1 min) (°C)	Final extension (7 min) (°C)
pgiC	pgiC16F	95	95	55	72	72
	pgiC21R
matK	670F, 193F44	95	95	52	72	72
	1246R44
rbcL-accD IGS	rbcL50F45	95	95	50	72	72
	accD79R44
trnK-rps16 IGS	trnKx146	95	95	53	72	72
	rps16 × 2F246
ycf6-psbM IGS	ycf6F47	95	95	54	72	72
	psbMR47
psbA-trnH IGS	psbAF48	95	95	55	72	72
	trnHR48