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. 2022 Mar 31;12:5495. doi: 10.1038/s41598-022-09390-8

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Sphere formation assay in colorectal cancer cells. (a, b) Representative pictures of sphere formation in DLD-1 (a) and SW620 (b) cells treated with siControl and siSmurf2. (c, d) The sphere formation rate were evaluated in DLD-1 (c) and SW620 (d) cells treated with siControl and siSmurf2.