Fig. 3.

Specific gene expression of HOS osteosarcoma cells for 2D monolayer, CSC and 3D bioprinted models. (A) Partial gene expression of cell cycle related pathways, including cell cycle, DNA replication, mismatch repair and one carbon pool by folate. (B) Partial gene expression of cell metabolism related pathways, including glycosaminoglycan biosynthesis chondroitin sulfate, valine leucine and isoleucine degradation, other glycan degradation and one carbon pool by folate. (C) Partial genes expression related to adherens junction. Red or green blocks represent up-regulation or down-regulation of gene expression compared to 2D control group. Colour depth represents fold change. All data are from n = 4 independent experiments.