Fig. 5.

Chemotherapeutic drugs cytotoxicity evaluation for three cell culture models (HOS). (A–H) Drug effect curves of eight clinical chemotherapeutics for osteosarcoma, including MTX (A), DXR (B), DDP (C), ERA (D), SORA (E), APAT (F), EVE (G) and CHQ (H). 2D models showed best sensitivity to MTX, DXR, DDP, ERA and SORA than the other two models. CSC models showed best sensitivity to APAT than the other two models. 3D models showed best sensitivity to EVE and CHQ than the other two models. Data are represented as mean ± SD (n = 3). (I) Corresponding IC50 calculated from the above eight drug effect curves respectively. MTX = methotrexate, DXR = doxorubicin, DDP = cisplatin, ERA = erastin, SORA = sorafenib, APAT = apatinib, EVE = everolimus, CHQ = chloroquine.