Figure 7.

Effects of DLGAP5 knockdown on the granulosa cell proliferation and apoptosis. Granulosa cells were transfected with si-NC or si-DLGAP5, and after siRNA transfection, (A) the mRNA expression level of DLGAP5 in the granulosa cells was determined by qRT-PCR; (B) the protein expression level of DLGAP5 in the granulosa cells was determined by western blot; (C) the cell viability of granulosa cells was determined by CCK-8 assay; (D) the cell proliferation of granulosa cells was determined by EdU assay, scale bar = 50 µm; (E) the cell apoptosis of granulosa cells was determined by TUNEL assay; scale bar = 50 µm. N = 3. **P < 0.01.