Figure 9.

Effects of DLGAP5 overexpression on the granulosa cell proliferation and apoptosis. Granulosa cells were transfected with LV-control or LV-DLGAP5, and after transfection, (A) the mRNA expression level of DLGAP5 in the granulosa cells was determined by qRT-PCR; (B) the protein expression level of DLGAP5 in the granulosa cells was determined by western blot; (C) the cell viability of granulosa cells was determined by CCK-8 assay; (D) the cell proliferation of granulosa cells was determined by EdU assay, scale bar = 100 µm; (E) the cell apoptosis of granulosa cells was determined by TUNEL assay, scale bar = 100 µm. *P < 0.05 and **P < 0.01.