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. 2022 Mar 18;16:824871. doi: 10.3389/fnins.2022.824871

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Copyright © 2022 Xu, Xiong, Xu, Wu, Li, Li, Jiang, Luo and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anxiety was shown in isoflurane-exposed old mice (n = 10). (A) The cross-time differences between the ISO-12.5 group and the CON group were not significant at age of 12.5 or 15 months. (B) The time stayed in the light box revealed not significant difference between the ISO-12.5 group and the CON group; however, a significant reduction of time stayed in the light box was shown in the ISO-12.5 group when these mice were at the age of 15 months (*p < 0.05). (C) The cross-time difference between the ISO-15 group and the CON group were not significant. (D) The time stayed in the light box was significantly reduced in the ISO-15 group compared with the CON group (*p < 0.05).