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The intersection number and spine density of the neuron in the cortex were reduced in isoflurane-exposed aged mice. (A) The Golgi staining picture to show neuron in the cortex in different groups. (B) The number of intersections at 150–200 μm from the cell soma was reduced in the cortex in the ISO-15 group compared with the CON group (n = 6, **p < 0.01). (C) The spine density was reduced in the cortex in the ISO-15 group compared with the CON group (n = 6, ***p < 0.001).
