Table 3.
Sequencing coverage and α-diversity indicators of gut microbiome.
| α-diversity | LD cycles | KO | CON | p-value | ||
|---|---|---|---|---|---|---|
| pKO | pLD | pINT | ||||
| Colonic content | ||||||
| Coverage, % | NLD | 99.89 ± 0.01 | 99.88 ± 0.01 | 0.912 | 0.740 | 0.328 |
| SL | 99.88 ± 0.01 | 99.90 ± 0.01 | ||||
| Chao 1 | NLD | 459.43 ± 3.92 | 370.93 ± 14.64 | 0.003 | 0.250 | 0.738 |
| SL | 437.40 ± 13.85 | 331.69 ± 10.43 | ||||
| ACE | NLD | 458.33 ± 7.75 | 330.68 ± 15.39 | 0.002 | 0.683 | 0.226 |
| SL | 423.86 ± 15.46 | 348.52 ± 14.09 | ||||
| Shannon | NLD | 5.86 ± 0.28 | 5.04 ± 0.03 | 0.007 | 0.589 | 0.857 |
| SL | 5.77 ± 0.22 | 4.85 ± 0.38 | ||||
| Simpson | NLD | 0.96 ± 0.01 | 0.90 ± 0.01 | 0.006 | 0.220 | 0.428 |
| SL | 0.95 ± 0.01 | 0.86 ± 0.04 | ||||
| Cecal content | ||||||
| Coverage, % | NLD | 99.89 ± 0.01 | 99.88 ± 0.01 | 0.109 | 0.683 | 0.878 |
| SL | 99.89 ± 0.02 | 99.87 ± 0.01 | ||||
| Chao 1 | NLD | 450.15 ± 13.68 | 395.16 ± 5.08 | <0.001 | 0.720 | 0.326 |
| SL | 466.46 ± 15.19 | 387.41 ± 3.58 | ||||
| ACE | NLD | 458.04 ± 7.58 | 387.71 ± 4.85 | <0.001 | 0.393 | 0.707 |
| SL | 454.87 ± 8.11 | 379.76 ± 2.10 | ||||
| Shannon | NLD | 5.79 ± 0.09 | 5.87 ± 0.03 | 0.039 | 0.433 | 0.006 |
| SL | 5.98 ± 0.11 | 5.58 ± 0.02 | ||||
| Simpson | NLD | 0.95 ± 0.01 | 0.95 ± 0.02 | 0.997 | 0.516 | 0.750 |
| SL | 0.96 ± 0.01 | 0.95 ± 0.01 | ||||
KO, Per2 gene knockout (Period2−/−) mice; CON, wild-type (Period2+/+) mice; NLD, normal light–dark cycle treatment of 12 h of light and 12 h of darkness; SL, short-light treatment of 8 h of light and 16 h of darkness. ACE, abundance-based coverage estimator. Statistical analyses were conducted using two-way ANOVA. p < 0.05, significant difference; p < 0.01 or p < 0.001, extremely significant difference; p > 0.05, without a difference. INT, the interaction between two factors. Results are presented as means ± SEM (n = 6 per group).