Figure 5.
Immunofluorescence signals merged with GFP show PTENK13R,A4 in PFA-fixed/pre-boiled EDTA-treated and methanol-fixed PC-3 cells. (A) Cells were detected with GFP (green) and an immunofluorescence assay with anti-PTEN rabbit monoclonal antibodies (clone 138G6) (magenta). Cells were chemically fixed with 4% PFA or cold methanol. Representative images of PC-3 cells expressing PTENK13R,A4-GFP. Scale bar: 20 µm. (B) PTENK13R,A4 at the plasma membrane was quantified as peak fluorescence intensities. Total fluorescence intensities were counted as 100 along the lines (40 µm). Average fluorescence intensities on both sides were represented as a box plot. A statistical analysis was performed using a one-way analysis of variance (ANOVA) with Tukey’s post hoc test. *p<0.05, **p<0.01, ***p<0.001.