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. 2022 Mar 18;10:807786. doi: 10.3389/fcell.2022.807786

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Copyright © 2022 Xu, Liu, Chen, Wang, Liu and Yi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Selection of representative genes differentially expressed in m⁵C cluster, related to metabolism and with prognostic significance. (A) Venn plot showing the overlapping analysis of genes that were differentially expressed (fold change >1.5 and p < 0.05) in two clusters, enriched in up-regulated metabolic pathways in each cluster, and had the prognostic significance. (B,C) Circus plot exhibiting the metabolic pathways up-regulated in each m⁵C cluster and those 13 genes with prognostic value were marked. Blue, up-regulated in cluster 1. Orange, up-regulated in cluster 2. (D) Volcano plot distributes the relative expressions 13 representative genes with log2 (fold change) and log10 (FDR). The reference is gene expression of cluster 1. Blue, up-regulated in cluster 1. Orange, up-regulated in cluster 2. (E–L) Kaplan–Meier overall survival curves of eight representative genes.