Figure 8.

CCL18-induced exosomal miR-760 activated the ARF6-mediated Src/PI3K/Akt signaling pathway

(A) Putative miR-760 binding sites within the ARF6 gene 5′UTR. (B) Schematic diagram of ARF61 5′ UTR-LUC and P1-P5 LUC plasmid. (C) The association between miR-760 and ARF6 was validated using luciferase reporter assay. ∗∗p < 0.01 compared with WT + miR-mimics. qRT-PCR was applied to determine the mRNA expression of ARF6 (D) and AMAP1 (E) in MCF-7 cells co-cultured with Exo-CCL18 or miR-760 mimics/inhibitor transfection. ∗p < 0.05, ∗∗p < 0.01, compared with mock. Western blot analysis was employed to detect the protein expression of (F) ARF6, AMAP1, and p-AMAP1, (G) EMT markers (E-cadherin and vimentin), as well as (H) the phosphorylated and total proteins of Src, PI3K, and AKT.