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. 2022 Mar 18;13:847581. doi: 10.3389/fimmu.2022.847581

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Copyright © 2022 Wang, Pan, Khan, Çil and Pineda

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sialidase-treated SFs show an inflammatory transcriptomic profile. SFs from naive mice were cultured and expanded ex vivo, RNA was isolated from non-treated control (NT) and C. perfringens sialidase treated (CP) SFs, then subjected to bulk RNA-Seq (75bp paired-end, 30M reads, n = 3). (A) Principal component analysis (PCA). (B) All detected genes are plotted as a volcano plot. Genes that passed a threshold of padj < 0.01 and |foldChange| > 2 are considered differentially expressed genes (DEGs), comparing sialidase-treated and non-treated SFs. Colour code, red: upregulated, blue: downregulated, in CP treatment. (C) Upregulated genes in sialidase treatment identified in (B) were used to perform KEGG pathway enrichment analysis. KEGG pathways are plotted in bubble chart with fold enrichment on x-axis and -log10 p-value on the coloured scale. The size of bubble is proportional to the number of DEGs in the given pathway.