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. 2022 Mar 18;13:847581. doi: 10.3389/fimmu.2022.847581

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Copyright © 2022 Wang, Pan, Khan, Çil and Pineda

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sialidase-treated synovial fibroblasts show an enhanced inflammatory response. (A) mRNA expression as detected by RNA-Seq from Figure 3 for MMP3, MMP9, MMP13, IL-6, CCL2, Myd88, Nfκbib, Tnfsf11 and Tnfrsf11b. (B–E) RNA was isolated from control (NT) and C. perfringens sialidase treated (CP) naïve SFs. Relative expression of the genes shown in (A) was assessed by RT-qPCR using the ΔΔCt method and actin as housekeeping gene. (B) IL-6 and CCL2, (C) MMP3, MMP9 and MMP13, (D) Myd88 and Nfκbib, and (E) Tnfsf11 and Tnfrsf11b. For (B–E), each dot represents one independent experiment, error bars represent SEM (n = 5), *p < 0.05, **p < 0.01 evaluated by Mann-Whitney test. NS, non-significant.