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Effect of TNF-α pretreatment on the inhibitory effect of HMR 3647 on oxidant production by PMN. PMN were pretreated with HSA (0.1%) or TNF-α (100 U/ml) for 30 min and then incubated with HMR 3647 or HBSS for 5 min before stimulation. Results are expressed as overall production of superoxide anion (mean ± standard error of the mean for three or four experiments). ∗, P < 0.05 for TNF-α versus HSA; ▵, P < 0.05 for HMR 3647- versus HBSS-treated cells.
