FIG. 7.

Effect of activators or inhibitors of protein kinases on roxithromycin uptake by HSA- or TNF-α-treated PMN. PMN were pretreated with various agents which alter protein phosphorylation for 10 min (A) or 45 min (B); they were then incubated with TNF-α or HSA for 30 min before roxithromycin was added for 5 min. Results are expressed as percent control uptake (mean ± standard error of the mean for 3 to 11 experiments [2 experiments for staurosporin {STAR}]). TP, tyrphostin; SB, SB 203580; PD, PD 098059. Control values (nanograms/2.5 × 10⁶ PMN/5 min) were 63 ± 5.7 (A) and 43 ± 5.6 (B). ∗, P < 0.05 versus control (HSA); $, P < 0.05 versus TNF-α alone; ▵, P < 0.05 for inhibitor versus inhibitor plus TNF-α.