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. 2022 Mar 5;298(4):101799. doi: 10.1016/j.jbc.2022.101799

Figure 3.

Figure 3

Electron density maps. Heme a (A) and loop I–II (B) in the R∗ state. The left panels of (A) and (B) show the structures of R∗ (green) overlaid with O∗ (blue) to highlight the annealing-induced structural transition. The 2FO–FC maps associated with the two structural elements in the R∗ state, shown in the right panels, are contoured at 1.25 σ and 1.5 σ, respectively. It is noted that there were no positive and negative features identifiable in the associated FO–FC density maps countered at 3.0 σ (not shown).