REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-ATGL	Proteintech	Cat# 10957-1-AP; RRID: AB_11182818
Rabbit polyclonal anti-STX11	Proteintech	Cat# 13301-1-AP, RRID: AB_2198214
Rabbit polyclonal anti-LAMP1	Proteintech	Cat# 21997-1-AP, RRID: AB_2878966
Rabbit polyclonal anti-LC3B specific	Proteintech	Cat# 18725-1-AP, RRID: AB_2137745
Rabbit polyclonal anti-perilipin2	Proteintech	Cat# 15294-1-AP
Mouse polyclonal anti-SIRT1	Proteintech	Cat# 60303-1-Ig, RRID: AB_2881417
Mouse monoclonal anti-Beta-Actin	Proteintech	Cat# Biotin-60008, RRID: AB_2883062
Mouse monoclonal anti-Beta-Tubulin	Proteintech	Cat# CL488-66240, RRID: AB_2883292
Rabbit polyclonal anti-PKAC	Cell Signaling Technology	Cat# 4782, RRID: AB_2170170
Rabbit polyclonal anti-phospho-PKAC(THr197)	Cell Signaling Technology	Cat# 4781, RRID: AB_2300165
Rabbit polyclonal anti-HA	Proteintech	Cat# 51064-2-AP, RRID: AB_11042321
Rabbit polyclonal anti-Myc	Proteintech	Cat# 10059-1-AP, RRID: AB_2282407
Mouse monoclonal anti-HA	Proteintech	Cat# 66006-2-Ig, RRID: AB_2881490
Mouse monoclonal anti-Myc	Proteintech	Cat# 60003-2-Ig, RRID: AB_2734122
Donkey anti-Mouse IgG(H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Invitrogen	Cat# A-21202, RRID: AB_141607
Donkey anti-Rabbit IgG(H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594	Invitrogen	Cat# A-21207, RRID: AB_141637
Donkey anti-Mouse IgG(H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594	Invitrogen	Cat# A-21203, RRID: AB_141633
Donkey anti-Rabbit IgG(H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647	Invitrogen	Cat# A-31573, RRID: AB_2536183
Donkey anti-Mouse IgG(H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647	Invitrogen	Cat# A-31571, RRID: AB_162542
Chemicals, peptides, and recombinant proteins
Oleic acid (OA)	Sigma-Aldrich	Cat# O1383
Lipo 8000 Transfection Reagent	Beyotime	Cat# C0533
BODIPY 493/503	Invitrogen	Cat# D3922
Fetal bovine serum	BI	Cat# 04-001-1A
DMEM	BI	Cat# 01-052-1A
Opti-MEM	GIBCO	Cat# 11058-021
DMEM (No-Glucose, No Phenol Red)	GIBCO	Cat# A1443001
Phosphate Buffered Saline (PBS)	BI	Cat# 02-024-1A
ER-Tracker Blue-White DPX	Invitrogen	Cat# E12353
Golgi-Tracker Red	Beyotime	Cat# C1043
DAPI	Beyotime	Cat# C1002
TRIzol reagent	Invitrogen	Cat# 11668019
1640 media	BI	Cat# 01-100-1A
Protease inhibitor cocktail	Sigma-Aldrich	Cat# P8340
chloroquine (CQ)	Sigma-Aldrich	Cat# 105M4035V
Isoproterenol (ISO)	Sigma-Aldrich	Cat# I5879
Critical commercial assays
Pierce BCA Protein Assay Kit	Thermo Fisher Scientific	Cat# 23225
EndoFree Plasmid Maxi Kit	QIAGEN	Cat# 12362
Triglyceride Assay Kit	Sigma	Cat# MAK266
Glycerol Assay Kit	Abcam	Cat# ab133130
Experimental models: Cell lines
THLE-2	ATCC	ATCC CRL-2706
HeLa	ATCC	ATCC CCL-2
HEK293T	ATCC	ATCC CRL-321
Oligonucleotides
Control siRNA: UUC UCC GAA CGU GUC ACG UTT	This paper	N/A
siSTX11: GCA UUA AGC GCG ACA CCA ATT	This paper	N/A
Recombinant DNA
pCDNA3.1-STX11-HA	This Paper	Expression construct generated in the lab
pCDNA3.1-STX11-Myc	This Paper	Expression construct generated in the lab
pCDNA3.1-ATGL-HA	This Paper	Expression construct generated in the lab
pCDNA3.1-ATGL-Myc	This Paper	Expression construct generated in the lab
pCDNA3.1-DGAT2-HA	This Paper	Expression construct generated in the lab
pEGFP-N2-STX11	This Paper	Expression construct generated in the lab
pEGFP-N2-ATGL	This Paper	Expression construct generated in the lab
pCDNA3.1-STX11ΔC-HA	This Paper	Expression construct generated in the lab
pCDNA3.1-ATGL-PT-Myc	This Paper	Expression construct generated in the lab
pCDNA3.1-ATGL-ΔPT-Myc	This Paper	Expression construct generated in the lab
pCDNA3.1-STX11-mcherry	This Paper	Expression construct generated in the lab
pGEX5X-3-STX11-GST	This Paper	Expression construct generated in the lab
pET30a-ATGL-MBP	This Paper	Expression construct generated in the lab
pCDNA3.1-ATGL-mcherry	This Paper	Expression construct generated in the lab
pET30a-ATGL-PT-MBP	This Paper	Expression construct generated in the lab
Software and algorithms
ImageJ	ImageJ	https://imagej.nih.gov/ij/
GraphPad Prism 8	GraphPad	www.graphpad.com
Adobe Illustrator 2020	Adobe	http://aotucad2.xmjfg.com/pg/230.html