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. 2022 Mar 18;9:871667. doi: 10.3389/fnut.2022.871667

FIGURE 6.

FIGURE 6

Outcome of EA extract on the expression patterns of Bax, BCL-2, Caspase-8, P53 and STAT3 in (A) MDA-MB-231 and (B) MDA-MB-436 cell lines. We note that EA extract induces an overexpression of the pro-apoptotic markers (Bax and Caspase-8) in comparison with their control, while anti-apoptotic marker Bcl-2 is inhibited. Furthermore, EA plant extract enhances the expression of p53 while inhibits phosphorylation of STAT3. GAPDH served as a control in this assay. Cells were treated with 100 and 200 μl/ml of EA extract for 48 h as explained in the materials and methods section. Values were corrected for the expression of the housekeeping protein GAPDH and presented as fold change of control. Data were analyzed using One-way ANOVA followed by Dunnett’s post hoc test. *P-value < 0.05 was considered for statistical significance. *p < 0.05, **p < 0.01, and ***p < 0.001. Data are presented as a percentage of treatment relative to the control (Mean ± SEM; n = 3).