Table 3.
Multilocus Sequence Typing of Complete Genes of Five Rickettsia asembonensis Specimens from Iquitos and Puerto Maldonado in Peru
| Peruvian R. asembonensis strain VGD7 |
Kenyan R. asembonensis strain NMRCii |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Conserved genes |
Variable genes |
Conserved genes |
Variable genes |
||||||||
| 17-kDa | gltA | sca4 | ompB | ompA | 17-kDa | gltA | sca4 | ompB | ompA | ||
| 8294D3 | #changes: nt/aa | 0/0 | 0/0 | 0/0 | 3/2 | 75/25 | 0/0 | 3/1 | 5/4 | 3/2 | 80/29 |
| %id: nt/aa | 100/100 | 100/100 | 100/100 | 99.94/99.88 | 98.54/98.54 | 100/100 | 99.77/99.77 | 99.84/99.60 | 99.94/99.88 | 98.45/98.31 | |
| 8556D1 | #changes: nt/aa | 0/0 | 0/0 | 0/0 | 4/2 | 75/25 | 0/0 | 3/1 | 5/4 | 4/2 | 80/29 |
| %id: nt/aa | 100/100 | 100/100 | 100/100 | 99.92/99.88 | 98.54/98.54 | 100/100 | 99.77/99.77 | 99.84/99.60 | 99.92/99.88 | 98.45/98.31 | |
| VGC2 | #changes: nt/aa | 0/0 | 0/0 | 0/0 | 3/2 | 75/25 | 0/0 | 3/1 | 5/4 | 3/2 | 80/29 |
| %id: nt/aa | 100/100 | 100/100 | 100/100 | 99.94/99.88 | 98.54/98.54 | 100/100 | 99.77/99.77 | 99.84/99.60 | 99.94/99.88 | 98.45/98.31 | |
| LER197 | #changes: nt/aa | 0/0 | 0/0 | 0/0 | 3/2 | 75/25 | 0/0 | 3/1 | 5/4 | 3/2 | 80/29 |
| %id: nt/aa | 100/100 | 100/100 | 100/100 | 99.94/99.88 | 98.54/98.54 | 100/100 | 99.77/99.77 | 99.84/99.60 | 99.94/99.88 | 98.45/98.31 | |
| LER205 | #changes: nt/aa | 0/0 | 0/0 | 0/0 | 3/2 | 75/25 | 0/0 | 3/1 | 5/4 | 3/2 | 80/29 |
| %id: nt/aa | 100/100 | 100/100 | 100/100 | 99.94/99.88 | 98.54/98.54 | 100/100 | 99.77/99.77 | 99.84/99.60 | 99.94/99.88 | 98.45/98.31 | |
The MLST analysis is presented in number of changes and percentage of identity at the nucleotide (nt) and amino acid (aa) level. MLST results for the conserved 17 kDa gene evidenced 100.0% of identity at both levels for comparisons against R. asembonensis strains VGD7 and NMRCii.
MLST, multilocus sequence typing.