Fig. 3.

Fat accumulation and carbonic anhydrase 3 (CA3) expression in human hepatoma HepG2 cells induced by culturing in medium containing oleic acid (OA). We tested the effects of acetazolamide (ACTZ) or ethoxyzolamide (ETZ), both CA3 inhibitors, on fat accumulation and CA3 expression. (A, B) Lipids in the HepG2 cells were stained using Oil Red O (A) and quantitatively analyzed by measuring dye absorbance at 540 nm (B). Magnification: 200×; scale bars: 500 μm. The data presented are mean ± SD (n = 6) absorbance relative to the control (no CA3 inhibitors). * P < 0.05, Mann–Whitney U‐tests compared with OA‐induced fat accumulation. (C) Western blot analysis of CA3 and GAPDH expression in HepG2 cells. GAPDH was used as an internal standard. (D) Band density was measured using imagej, calibrated against GAPDH. Data presented are mean ± SD density (n = 6).