Figure 2.

A common ancestry between oxyntocardiac, cardiac-type, and specialized epithelium in BE. (A) H&E with areas of interest outlined. (B) MUC5AC is extensively expressed on all surface foveolar cells. (C) H⁺K⁺ATPase⁺ parietal cell–containing glands and (D) MUC2⁺ goblet cell glands on the same section. (Ci–iii) and (Di–iii) show high-power magnification images of C and D, respectively, with adjacent cytochrome c oxidase staining (Cii, Dii) and postmicrodissection images (Ciii, Diii). (E) PCR sequencing showed a common, complex m.303-311 Cins mutation in the MT-OHR region of the mitochondrial genome present in oxyntocardiac MUC5⁺MUC2^– surface (1) and H⁺K⁺ATPase⁺ base (2) glands as well as MUC5AC⁺MUC2⁺ specialized glands (3 and 4) and a MUC5AC⁺MUC2^– cardiac-type gland (5). (F) The stroma and 1 other cardiac-type MUC5AC⁺MUC2^– gland (6) sequenced were wild type. All microdissected glands are traced by dotted outlines.