Supplementary Table 3.
Characteristics of the Primary Antibodies Used for IHC
| Antibody | Source | Host/isotype | Dilution FFPE | Dilution FF | Secondary | Tertiary |
|---|---|---|---|---|---|---|
| Pepsinogen | Abcam | Mouse/IgG1 | 1:700 | N/A | Goat anti-mouse | Streptavidin AP/blue |
| H+K+ATPase | DAKO | Mouse/IgG1 | 1:1000 | 1:4000 | Goat anti-mouse | Streptavidin HRP/DAB |
| MUC5AC | Abcam | Mouse/IgG1 | 1:500 | 1:50/1:100 | Goat anti-mouse | Streptavidin HRP/DAB |
| MUC2 | Thermo Fisher Scientific | Mouse/IgG1 | 1:500 | 1:200 | Goat anti-mouse | Streptavidin AP/blue |
| Defensin6α | Sigma | Rabbit/polyclonal | 1:5000 | N/A | Swine anti-rabbit | Streptavidin AP/blue |
| Defensin5α | Abcam | Mouse/IgG1 | N/A | 1:100 | Rabbit anti-mouse | Streptavidin HRP/DAB |
| MUC6 | Abcam | Mouse/IgG1 | 1:500 | N/A | Goat anti-mouse | Streptavidin HRP/DAB |
NOTE. Antibody sources, dilutions, and processing used in this article.
FF, fresh frozen; N/A = not applicable (antibody not used for this purpose).