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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: J Integr Plant Biol. 2021 Dec;63(12):2020–2030. doi: 10.1111/jipb.13181

Figure 5. Characterization of P100 EVs using iodixanol gradients.

Figure 5.

(A) P100 fraction obtained after 100,000×g centrifugation was allowed to float into an overlayed iodixanol gradient by top loading. Sup, Supernatant. (B) Six fractions were collected and analyzed by Western blot, showing the TET8-positive EVs enriched in a single fraction (F3) with TET8 native antibody. (C) Representative TEM images of F3 fraction in (B). Scale bars, 500 nm. (D) Histogram for the size distribution of EVs in F3 fraction in (C) (284 vesicles analyzed).