TABLE 1.
Characteristics of PZA-resistant clinical isolates of M. tuberculosis
| Straina | Resistance to other drugsc | PZA MIC (μg/ml) | PZase activity |
pncA mutation(s)b
|
|
|---|---|---|---|---|---|
| Nucleotide change(s) | Amino acid change(s) | ||||
| 9869 | INH, RMP, SM | >300 | − | −11 promoter mutation A→G | |
| 10347 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift∗ |
| 9131 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 11041 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 11135 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 9721 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 9769 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 11823 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 9132 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 9579 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 10800 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 10350 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 11243 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 9811 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 9004 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 10348 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 10257 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 10003 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 11743 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 10611 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 9155 | Monoresistant | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| 9953 | INH, RMP, EMB | >300 | − | C to A at 418, 8-bp deletion at 446 | Arg140→Ser, frameshift |
| W39117 | INH, RMP, EMB, Oflox | >900 | − | A to G at 29 | Gln10→Arg∗ |
| H3628 | INH, RMP | >900 | − | A to C at 35 | Asp12→Ala |
| K10451 | NT | >500 | − | C to A at 137 | Ala46→Glu |
| K10437 | NT | >500 | − | C to T at 137 | Ala46→Val |
| F29684 | INH, RMP, EMB | >900 | − | C to T at 137 | Ala46→Val |
| W5457 | NT | >900 | − | C to T at 137 | Ala46→Val |
| A7259 | INH, RMP, SM | >900 | − | C to T at 137 | Ala46→Val |
| A7183 | INH, RMP, SM | >900 | − | C to T at 137 | Ala46→Val |
| 8989 | NT | >900 | − | C to T at 137 | Ala46->Val |
| K10552 | NT | >100 | − | A to C at 158 | Asp53→Ala∗ |
| PZA-R9717 | Monoresistant, in vitro mutant | >900 | − | G to A at 203 | Trp68→stop∗ |
| A7157 | INH, RMP, SM | >900 | − | T to G at 213 | His71→Glu∗ |
| A7153 | INH, RMP, EMB, SM | 300 | − | A to G at 245 | His82→Arg∗ |
| K10400 | NT | >500 | − | T to G at 254 | Leu85→Arg |
| T63168 | INH, RMP, EMB, SM | >900 | − | G to A at 289 | Gly97→Ser∗ |
| M52997 | INH, RMP, EMB, SM | >900 | − | G to A at 289 | Gly97→Ser |
| 10426 | INH, RMP, SM | >300 | − | C to T at 401 | Ala134→Val |
| K10551 | NT | >500 | − | G to C at 413 | Cys138→Ser |
| K10428 | NT | >500 | − | G to A at 415 | Val139→Met∗ |
| W76757 | INH, RMP, EMB, SM | >300 | − | A to C at 422 | Gln141→Pro |
| K10447 | NT | >500 | − | T to G at 464 | Val155→Gly |
| 099-3-CIP8 | RMP, Oflox | 300 | − | C to A at 503 | Thr168→Asn∗ |
| 10467 | INH, RMP, SM | >300 | − | G insertion at 52∗ | Frameshift |
| 11627 | SM | >300 | − | A insertion at 193∗ | Frameshift |
| A7156 | INH, RMP, SM | >900 | − | A insertion at 193 | Frameshift |
| K10429 | NT | >500 | − | G deletion at 301∗ | Frameshift |
| K10452 | NT | >500 | − | C deletion at 341∗ | Frameshift |
| H2374 | RMP | >900 | − | G insertion at 420∗ | Frameshift |
| H1033 | INH, RMP, SM, Oflox | >900 | − | G insertion at 420 | Frameshift |
| K10436 | NT | >100 | − | GG insertion at 428∗ | Frameshift |
| T61823 | INH, RMP, SM | >900 | − | GG insertion at 420, T to G at 385 | Val130→Gly∗ |
| 11552 | INH | >300 | − | No mutation | |
| M49586 | NT | >900 | − | No mutation | |
| F57636 | INH, RMP | >900 | − | No mutation | |
| 9739 | NT | 300 | + | No mutation | |
| 10274 | NT | 100 | + | No mutation | |
| 11830 | NT | 100 | + | No mutation | |
a
Strain designations with only numbers indicate strains from Quebec, Canada Strain designations starting with K indicate strains from South Korea, and strain designations starting with other letters indicate U.S. strains.
c
SM, streptomycin; Oflox, ofloxacin; NT, not tested.