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. 2022 Mar 31;55(3):142–147. doi: 10.5483/BMBRep.2022.55.3.090

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Fig. 2 — Binding capacity of K312 to H9 hESCs decreases in a differentiation-dependent manner. (A) H9 hESCs were differentiated into embryoid bodies (EBs) for 9 days, and flow cytometry analysis reveals the binding of K312 to cells, in comparison with that of antibodies against stage-specific embryonic antigen (SSEA)-3 and Tra-1-60. (B) Immunoblotting analysis of the expression of the target of K312, PSC surface markers (E-cadherin and EpCAM), and transcription factors (Oct4, Nanog, and Sox2) on the indicated days. (C and D) Downregulation of the target of K312 by retinoic acid (RA)-induced differentiation of H9 hESCs. Binding of K312 to cells and protein expression on each day of differentiation were analyzed by flow cytometry and immunoblotting, respectively.