Fig. 3.

K312 distinguishes highly pluripotent cells from undifferentiated H9 hESCs. (A) H9 hESCs stained with K312 separate into K312-high and -low cell populations by cell gating with an isotype control antibody. (B) Immunoblotting analysis of the expression of the target of K312, Oct4, Nanog, and E-cadherin in K312-high and -low cells. (C and D) Single K312-high and -low cells were cultured for 1 week to form colonies. Colonies were analyzed by alkaline phosphatase staining (C) and counted for quantitative comparison (D). ***P < 0.001, Student’s t-test.