FIGURE 1.

Knockdown of rap1 results in altered targeting of integrin β. (A) Immunofluorescence analysis of tangential sections (A) or surface sections (B) of control (sns >> control RNAi) or rap1 knockdown nephrocytes (sns>>rap1 RNAi) is shown. Knockdown in nephrocytes was accomplished by employing sns-GAL4 and two different RNAi hairpins (#20761 and #110757). Nephrocytes of wandering third instar larvae were dissected, and immunofluorescence analysis was performed with antibodies specific for integrin β (green) and Sns (red). Merged images and higher magnifications of the marked area (detail) are shown. Scale bars in (A,B): 10 µm, n = 3. (C) The intensity of integrin fluorescence at the cell membrane compared to the cytosol was evaluated for all genotypes shown in (A) and presented as a box plot with minimum and maximum in arbitrary units (AU). The control is marked in pink. *** indicates a p-value < 0.001.