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. 2022 Mar 18;10:790365. doi: 10.3389/fcell.2022.790365

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Copyright © 2022 Maywald, Picciotto, Lepa, Bertgen, Yousaf, Ricker, Klingauf, Krahn, Pavenstädt and George.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Imbalance of d-rap1 activity leads to loss of slit diaphragms and lacunae. (A) TEM analysis of dissected control nephrocytes (sns>>control OE), nephrocytes with overexpression of Drosophila wild-type rap1 (sns>>d-rap1 WT), of Drosophila dominant-negative rap1 (sns>>d-rap1 N17), or of Drosophila constitutively active rap1 (sns>>d-rap1 V12). Knockdown in nephrocytes was accomplished by employing sns-GAL4. White arrows exemplarily indicate slit diaphragms. Scale bars: upper panel 500 nm, lower panel zoom compared to the upper panel 2×. n = 3. (B) Statistical evaluation of the number of slit diaphragms (SD) per µm basement membrane of the genotypes shown in (A) depicted as a box plot with minimum and maximum. The control is marked in pink. **p < 0.01, ***p < 0.001.